Abstract

A new, efficient method to biologically and pharmaceutically important (E)-4-methoxy-N?-2,3,4-trimethoxybenzylidene)benzohydrazide monohy drate (1c) was prepared by reacting 4-methoxybenzohydrazide (1a) and 2,3,4-trimethoxybenzaldehyde (1b) in the presence of a concentrated hydrochloric acid as an efficient catalyst. The synthesized compound was evaluated for antimicrobial activity against Gram-positive pathogen Staphylococcus aureus, Gram-negative pathogen Escherichia coli, and Aspergillus niger as fungi. The compound exhibited good anti-bacterial and antifungal activity at Minimum Inhibitor Concentration level. The antioxidant activity was determined by DPPH method and that compound possesses best antioxidant activity. Further, the synthesized compound was evaluated for their cytotoxicity against breast cancer cell line (MCF-7). The result showed that the synthesized compound is non-toxic in nature.